LOCUS       40924_5914        4977 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBARST DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Abe A, Elegado EB, Sone T.
  TITLE     Construction of pDESTR, a GATEWAY vector for gene disruption in 
            filamentous fungi
  JOURNAL   Curr. Microbiol. 52 (3), 210-215 (2006)
  PUBMED    16479358
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Sone T, Abe A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2005) Teruo Sone, Hokkaido University, Graduate 
            School of Agriculture; Kita 9 Nishi 9, Kita-ku, Sapporo, Hokkaido 
            060-8589, Japan (E-mail:sonet@chem.agr.hokudai.ac.jp, 
            Tel:81-11-706-2502, Fax:81-11-706-4961)
REFERENCE   3  (bases 1 to 4977)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4977)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr. 
            Microbiol."; date: "2006"; volume: "52"; issue: "3"; pages: 
            "210-215"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-NOV-2005) Teruo Sone, Hokkaido University, Graduate School of 
            Agriculture"; volume: " Kita 9 Nishi 9, Kita-ku, Sapporo, Hokkaido 
            060-8589, Japan (E-mail:sonet@chem.agr.hokudai.ac.jp, 
            Tel:81-11-706-2502, Fax"; pages: "81-11-706-4961"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(233..821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(995..1852)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1853..1957)
                     /label=AmpR promoter
     promoter        2063..2164
                     /label=TRP1 promoter
     promoter        2259..2618
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             2641..3189
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     protein_bind    3270..3394
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3419..3449
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             3503..4159
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             4504..4806
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(4850..4974)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
       61 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      121 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      181 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      241 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      301 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      361 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      421 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      481 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      541 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      601 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      661 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      721 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      781 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
      841 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
      901 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
      961 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1021 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1081 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     1141 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1201 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1261 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     1321 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     1381 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     1441 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     1501 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     1561 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     1621 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     1681 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     1741 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     1801 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     1861 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     1921 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     1981 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     2041 ggccctttcg tcttcaagaa ttaattcggt cgaaaaaaga aaaggagagg gccaagaggg
     2101 agggcattgg tgactattga gcacgtgagt atacgtgatt aagcacacaa aggcagcttg
     2161 gagtatgtct gttattaatt tcacaggtag ttctggtcca ttggtgaaag tttgcggctt
     2221 gcagagcaca gaggccgcag aatgtgctct agactcgaca gaagatgata ttgaaggagc
     2281 actttttggg cttggctgga gctagtggag gtcaacaatg aatgcctatt ttggtttagt
     2341 cgtccaggcg gtgagcacaa aatttgtgtc gtttgacaag atggttcatt taggcaactg
     2401 gtcagatcag ccccacttgt agcagtagcg gcggcgctcg aagtgtgact cttattagca
     2461 gacaggaacg aggacattat tatcatctgc tgcttggtgc acgataactt ggtgcgtttg
     2521 tcaagcaagg taagtgaacg acccggtcat accttcttaa gttcgccctt cctcccttta
     2581 tttcagattc aatctgactt acctattcta cccaagcaaa gcttcgatta ggaagtaacc
     2641 atgagcccag aacgacgccc ggccgacatc cgccgtgcca ccgaggcgga catgccggcg
     2701 gtctgcacca tcgtcaacca ctacatcgag acaagcacgg tcaacttccg taccgagccg
     2761 caggaaccgc aggagtggac ggacgacctc gtccgtctgc gggagcgcta tccctggctc
     2821 gtcgccgagg tggacggcga ggtcgccggc atcgcctacg cgggcccctg gaaggcacgc
     2881 aacgcctacg actggacggc cgagtcgacc gtgtacgtct ccccccgcca ccagcggacg
     2941 ggactgggct ccacgctcta cacccacctg ctgaagtccc tggaggcaca gggcttcaag
     3001 agcgtggtcg ctgtcatcgg gctgcccaac gacccgagcg tgcgcatgca cgaggcgctc
     3061 ggatatgccc cccgcggcat gctgcgggcg gccggcttca agcacgggaa ctggcatgac
     3121 gtgggtttct ggcagctgga cttcagcctg ccggtaccgc cccgtccggt cctgcccgtc
     3181 accgagatct gatccgtcga cctgcagatc gttcaaacat ttggcaataa agtttcttaa
     3241 gattgaatcc tgttgccggt cttgcgatca caagtttgta caaaaaagct gaacgagaaa
     3301 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     3361 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     3421 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
     3481 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     3541 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     3601 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     3661 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     3721 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     3781 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     3841 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     3901 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     3961 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     4021 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     4081 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     4141 tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta
     4201 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     4261 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     4321 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     4381 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     4441 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     4501 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     4561 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     4621 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     4681 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     4741 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     4801 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     4861 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     4921 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgat
//
